Microwave-assisted Weak Acid Hydrolysis of Proteins
نویسندگان
چکیده
منابع مشابه
Microwave-assisted method for microassay of proteins using bicinchoninic acid.
A microwave-assisted microassay has been developed for estimation of proteins, using bicinchoninic acid (BCA). The protocol described here is rapid with a reaction time of merely 95 sec, requires lesser amounts of BCA and other reagents, and tolerates larger or comparable concentration of various interfering substances.
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The complete hydrolysis of konjac glucomannan (KGM) with an acid or enzyme generally takes a long time. To accelerate KGM hydrolysis without diminishing the conventional quality, a diluted acid hydrolysis of KGM with sulfuric acid was conducted using a microwave digestion system. The optimum conditions of microwave-assisted acid hydrolysis for KGM were: 10 mL of 0.25 M sulfuric acid, hydrolysis...
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ژورنال
عنوان ژورنال: Mass Spectrometry Letters
سال: 2012
ISSN: 2233-4203
DOI: 10.5478/msl.2012.3.2.47